Clive Palmer's company Queensland Nickel donated almost $290,000 to his troubled political party just two weeks before sacking 237 workers from its Townsville refinery.
The company announced the redundancies on Friday after the Queensland government refused Mr Palmer's December request that it act as a guarantor for a $35 million loan.
Sacked workers: 'pretty heartbreaking'
Workers sacked at Clive Palmer's Townsville nickel refinery on Friday say they "can see the place folding in six months anyway". Nine News
But a new Electoral Commission of Queensland document shows the company donated $288,516 to the Palmer United Party on December 31.
The "Special Reporting Event Return" document is signed by managing director Clive Mensink, who is Mr Palmer's nephew.
Mr Palmer is now one of only two PUP members in Federal Parliament after senators Glenn Lazarus and Jacqui Lambie abandoned the party. Opinion polls show public support for PUP has waned significantly since the high water mark of the 2013 election.
Queensland Nickel has donated millions of dollars to PUP over the last couple of years, records show.
But in making the redundancy announcement on Friday, Mr Mensink blamed Premier Annastacia Palaszczuk and her government.
"The Queensland government has made it clear despite the nickel price being the lowest in 15 years it has no interest in assisting Queensland Nickel in providing continued employment for over 800 families in Townsville," he said.
"Because of the current nickel price and because of the failure of our own government to offer any support for our company's continued operations in Townsville, today Queensland Nickel has been forced to make 237 workers redundant."
When the government rejected Mr Palmer's request, Treasurer Curtis Pitt said Queensland Nickel was an "undue risk" for taxpayers.
Acting Premier Jackie Trad has rejected the suggestion government was in any way responsible for the job losses.
She said the government tried to work with the company but a request for access to the full financial statements of Mr Palmer's businesses was not responded to.
"We could not in all good conscience hand over money to a private company without full financial due diligence."