Prince William, Duke of Cambridge plants an Aleppo Pine seedling derived from seeds gathered after the battle of Lone Pine at Gallipoli, on ANZAC Day on April 25 2014 in Canberra, Australia.

Prince William, Duke of Cambridge plants an Aleppo Pine seedling derived from seeds gathered after the battle of Lone Pine at Gallipoli, on ANZAC Day on April 25 2014 in Canberra, Australia. Photo: REUTERS/Mark Graham/Pool

A lone pine descended from a tree at the bloody battlefield of Gallipoli; an Australian family's loss; and a now a double royal connection.

Following the national Anzac Day ceremony at the Australian War Memorial in Canberra on Friday, the Duke and Duchess of Cambridge planted an Aleppo Pine sapling derived from seeds gathered after the battle of Lone Pine.

After laying floral wreaths at Tomb of the Unknown Australian Soldier, war memorial director Brendan Nelson and Rear Admiral Ken Doolan and their wives Gillian Adamson and Elaine Doolan accompanied the couple to the tree planting site.

Australian War Memorial Director Brendan Nelson (R) speaks with Catherine, Duchess of Cambridge and Prince William, Duke of Cambridge and Australian War Memorial Chairman Ken Doolan (L) looks on after planting an Aleppo Pine seedling derived from seeds gathered after the battle of Lone Pine at Gallipoli.

Australian War Memorial Director Brendan Nelson (R) speaks with Catherine, Duchess of Cambridge and Prince William, Duke of Cambridge and Australian War Memorial Chairman Ken Doolan (L) looks on after planting an Aleppo Pine seedling derived from seeds gathered after the battle of Lone Pine at Gallipoli. Photo: REUTERS/Mark Graham/Pool

Kate wore a grey tweed coat dress, midnight blue head piece, and a poppy broach that Emma, the wife of Australian Victorian Cross recipient Ben Roberts-Smith, gave her at a reception in Canberra last night.

For Prince William, who served in Britain's Royal Air Force for more than seven years, the tree had a special family connection.

Prince Henry, the Duke of Gloucester - who later went on to serve as Australia's governor-general after the Second World War - planted the original lone pine at the war memorial in October 1934.

Catherine, Duchess of Cambridge, uses a shovel to place dirt at the base of a tree as her husband Britain's Prince William watches with officials at the Australian National War Memorial.

Catherine, Duchess of Cambridge, uses a shovel to place dirt at the base of a tree as her husband Britain's Prince William watches with officials at the Australian National War Memorial. Photo: REUTERS/Mark Graham/Pool

That tree which is coming to the end of its natural life, was propagated from a pine cone from Gallipoli which Australian Lance Corporal Benjamin Smith, sent home to his mother Jane McMullin.

It commemorated his brother Mark who died on August 6, 1915 in the battle of Lone Pine, one of the bloodiest in the campaign with more than 2000 Australian casualties in four days.

Turkish troops had cut down pine trees for their trenches and only a solitary one remained when the battle began.

Britain's Catherine, the Duchess of Cambridge, holds her son Prince George as they prepare to board a plane with her husband Prince William (not pictured) to depart Canberra April 25, 2014. Click for more photos

Duke and Duchess of Cambridge in Canberra on Anzac Day

Britain's Catherine, the Duchess of Cambridge, holds her son Prince George as they prepare to board a plane with her husband Prince William (not pictured) to depart Canberra April 25, 2014. Photo: Phil Noble

Mrs McMullin raised two seedlings - the other was planted at Inverell in NSW's north, where her sons enlisted.

For decades the Yarralumla Nursery has been collecting and propagating seeds and distributing saplings.

The duke was given a photograph of Prince Henry's tree planting framed in wood from the war memorial's lone pine and commemorative wooden boxes as well.

Former Liberal MP Sophie Mirabella and her young daughters were among the crowd lining the path to see the couple.

They had a sign which read "Wangaratta honours their service". Her daughter Kitty, 3 and a half yelled out to Kate that she was very beautiful.

The royal couple made a surprise appearance at the dawn service earlier.

They head home with baby George in afternoon bringing an end to their Australian tour.