The pride of Australia's batting has taken a major hit ahead of Friday's first Test after being bowled out for 235 in reply to India A's 451 on the third day of their three-day warm-up game.
Shane Watson top-scored with 84 for Australia at Chennai's Guru Nanak College Ground while Ed Cowan (40), Moises Henriques (33) and Matthew Wade (44) also made starts.
India A captain Gautam Gambhir has enforced the follow-on and Australia will bat for half an hour before lunch on Monday.
Apart from Peter Siddle's run-out, all of Australia's wickets fell to spinners Rakesh Dhurv (5-51) and Jajaj Saxena (4-61).
Australia had resumed on 4-131.
Uncapped all-rounder Henriques hit three sixes and two fours in his 33 before the big right-hander was lbw to Dhurv.
Siddle was run out for two attempting a third run at 6-182.
Indian reporters seated in a media tent side-on to the pitch said the ball had touched the rope and Siddle should have been awarded a boundary.
India A's spin duo also removed Mitchell Starc (11), Nathan Lyon (duck) and Xavier Doherty (seven) before Wade hit out and was caught on the boundary.
Australia have announced 19-year-old spinner Agar will head home on Monday night to resume state duties with Western Australia, ruling him out of a shock call-up for the first Test.
The left-armer claimed 3-107 in India A's first innings in just his third first-class game.
Australia's four-Test series against India starts on Friday at Chennai's Chidambaram Stadium.