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Perth: Australia's experienced Hopman Cup team couldn't replicate their younger compatriots from earlier in the day with Lleyton Hewitt and Jarmila Wolfe both losing to their Czech Republic opponents at the Perth Arena.
The Australian Green team of Nick Kyrgios and Daria Gavrilova beat Germany's Alexander Zverev and Sabine Lisicki 3-0 to open the Hopman Cup earlier on Sunday.
But the Gold team flopped in the evening with Hewitt and Wolfe (formerly Gajdosova) losing their singles matches to Jiri Vesely and Karolina Pliskova.
The night began with Hewitt's first match on his farewell summer.
He lost the opening set but showed his trademark fight to win the second set in a tie-break only to lose the decider to his 22-year-old world No. 41 opponent 6-4.
Given it was his first singles match since losing in the second round at the US Open to Bernard Tomic in five sets, Hewitt was happy to get the tough three-setter under his belt despite the 6-3, 6-7, 6-4 loss.
"I got better as the match went on," Hewitt said.
"I hadn't played a competitive singles match since the US Open so it was a really good hit out for me.
"I thought the standard was pretty good, especially in the second and third sets, and he's a quality player with a big game. I think I'll be better for the run."
Wolfe then needed to beat world No. 11 Karolina Pliskova, but went down in straight sets.
Pliskova broke in the 12th game of the first set to take it out but the Australian raced to a 3-1 lead in the second.
However, she lost the next five straight games as the Czech 23-year-old won 7-5, 6-3 in 64 minutes.
The Australian pair then lost the mixed doubles in straight sets 6-2, 7-5 to complete a clean sweep for the Czechs.
The second day of the 2016 Hopman Cup will begin with Ukraine's Alexandr Dolgopolov and Elina Svitolina playing the USA's Jack Sock and Serena Williams.
In the evening, Great Britain's Andy Murray and Heather Watson tackle France's Kenny de Schepper and Caroline Garcia.