Canberra-based company Aspen Medical has launched a 24-hour ambulance service in south Texas.
The service was formally launched by ACT Chief Minister Andrew Barr and Aspen co-founder Glenn Keys during a trade mission to the US.
Four ambulances with provide transfers to the San Antonio hospital with the service likely to expand into other areas of Texas later this year.
Mr Keys said the ambulances would provide reliable and affordable care and have "a positive impact on these communities and save lives".
The company has had an office in San Antonio since 2008 and has built a reputation for providing health care in remote or challenging environments.
Mr Barr said the company was a Canberra success story and he was proud to see the business securing contracts abroad.
"It is my hope that Aspen Medical will inspire other local businesses and entrepreneurs in our city to dream big and to use their talents, creativity and innovation to make a difference both at home and abroad," he said.
Last year the Australian government contracted the Deakin-based firm to staff a 100-bed British-built Ebola treatment centre in Sierra Leone, at a cost of up to $20 million.
Canberra Business Chamber chief executive Robyn Hendry said Aspen had grown to be a major international player since launching in 2003.
"Aspen Medical is already doing well in the United States and it plans to extend its ambulance service operation across other counties," she said.
"During our time in the United States, all the local businesses over here with us have been well received and I believe a number of opportunities for ACT businesses could emerge from this trip."
The five-day trade mission ended on Friday with 26 local companies and representatives of the Canberra Business Chamber travelling to San Francisco, Silicon Valley and Austin.