There will be no special treatment for AFL players, with the ACT government declaring any Melbourne-based teams would need to go into quarantine for 14 days if they come to Canberra.
But Chief Minister Andrew Barr said teams from the other states, besides Victoria, could play at Manuka Oval.
The door's also ajar for Victorian clubs to play in Canberra once they've been away from their home state for required two weeks.
All 10 Victorian clubs will base themselves in Queensland, Western Australia or NSW following this weekend's round of games.
The AFL announced their fixtures for rounds six and seven on Friday, which begins with Geelong hosting the Brisbane Lions at the SCG next Thursday.
Barr announced the introduction of the quarantine measures on Friday with anyone who breaks them facing an $8000 fine.
That will also apply to any AFL players who live in the various "hotspots" throughout Melbourne or their teammates.
Canberra was being mooted as a potential venue to host an AFL bubble, but at the moment the AFL has gone with other options.
There are currently no confirmed cases of the coronavirus in the ACT, with the new measures designed to keep it that way.
When asked about the potential for the AFL to bring games to Canberra, Barr said they would need to follow the same rules as everyone else.
"Given what we've just announced, none of the Victorian teams - if any of the players or support staff were from greater metropolitan Melbourne, particularly the hot spots - would be able to come into the ACT without the 14-day quarantine period," he said.
"If they're coming out of Melbourne - obviously there are other AFL teams that are Queensland, South Australian, West Australian and Sydney-based - but there'd be no exemption for AFL players from what we've just announced."
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Barr's comments come as the ACT government considered increasing the number of fans allowed at Canberra Stadium.
ACT chief health officer Kerryn Coleman inspected the stadium on Wednesday and was confident in the measures both the Canberra Raiders and the ACT Brumbies had in place for their games there over the weekend.
The Raiders hosted St George Illawarra on Friday night, while the Brumbies play the Melbourne Rebels on Saturday.
Crowds were restricted to 1500 for both games, but Coleman was confident of increasing that for next weekend when the Raiders host the Melbourne Storm on the Saturday night.
"I visited on Wednesday and got a little tour of the stadium and checked out the measures that they're putting in place, and I'm very confident that they're well thought through and very safe," Coleman said.
"I'll be looking forward to the demonstration of how that goes and we're anticipating being able to increase crowd numbers next weekend."