Greater Brisbane is no longer to be considered a COVID-19 hotspot in the ACT, from 2pm Monday.
It means about 4800 ACT residents and non-residents who had been in the Queensland capital from January 2 can leave quarantine.
On Friday, anyone in Canberra who had visited Brisbane in the week prior was ordered into 14 days' quarantine.
The city was declared a coronavirus hotspot after a cleaner at a quarantine hotel was diagnosed with the UK variant of COVID-19.
There are fears the strain is far more contagious than other variants of the virus.
Chief health officer Kerryn Coleman said she decided to remove the hotspot classification after consulting with her Queensland counterpart about the case.
"The early signs are positive however we will not know if the variant has spread further until at least a full 14 days after the last day the infectious case was in the community," Dr Coleman said.
Dr Coleman said people who went into quarantine after visiting Queensland would not have to undertake a COVID-19 test, but would need to monitor for symptoms and get tested if they started to experience even the mildest of symptoms.
About 4800 people told ACT Health they had been in Greater Brisbane and subsequently went into quarantine in the ACT.
"We want to thank the ACT community for doing the right thing to keep our community safe," Dr Coleman said.
"I would like to thank all those affected, including our local hotels and accommodation providers, for your co-operation and patience during this time."
The lifting of restrictions came as Queensland Premier announced the snap lockdown of greater Brisbane would end on Monday night.