The Australian-led operation look over 80 Australians, Afghan visa holders, and New Zealanders from Kabul to the United Arab Emirates on Friday evening. Around 240 Australians and visa holders have now been evacuated in total.

The flight was the third carrying Australians out of Kabul since it fell into the hands of the Taliban a week ago.

Government frontbencher Stuart Robert warned the situation on the ground remained fluid.

"It is a very difficult situation in Kabul. It's difficult at the best of times ... let alone now with streets choked, and issues of course with the change of power. It's very uncertain, it is certainly dangerous," he told reporters on Saturday.

A group of 94 Australians and Afghans seeking refuge were quarantining in Perth after landing in the early hours of Friday morning. They were the first to arrive in Australia since the Taliban's lighting advance.

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But Prime Minister Scott Morrison has already conceded many of the Australian visa holders scattered across Afghanistan may be unable to reach Kabul.

And those in the capital were also struggling to access Hamid Karzai Airport, their only available exit point, as the Taliban blocked entrances in an apparent attempt to prevent Afghan nationals from leaving. Foreign Minister Marise Payne on Friday revealed injuries had been suffered in the chaos, including by Australian passengers.

It came just days after seven people were killed in distressing scenes at the airport, as thousands of Afghans attempted to board an American rescue plane. And amid ongoing danger on its parameter, Mr Robert urged Australians and visa holders to maintain contact with the Department of Foreign Affairs and Trade.

"For those who are still in Afghanistan, the message remains constant: keep in touch with DFAT, and make sure your details are recorded with [them]. DFAT will co-ordinate with you whatever the next steps are," he said.

Further rescue flights were planned, but Mr Morrison has warned expected poor weather and limited places on the runway would hamper efforts.

The Taliban's advance, which shocked international observers with its speed, has plunged hundreds of Afghan interpreters who aided Australian forces since the 2001 invasion into grave danger.

Amnesty International has accused the group of massacring nine Hazara men in Ghazni Province, in the country's south-east, last month. It warned the brutal killings were likely "a tiny fraction" suffered by the persecuted minority.

The federal government evacuated embassy staff in May, and has faced criticism for not doing more to protect those who worked with the ADF.

Amid reports of Taliban reprisals against Afghans who worked with coalition forces, Mr Morrison last week conceded some would die at the hands of the hardline Islamist group. Labor frontbencher Mark Butler said the government had been too slow to ensure their safety.

"I fear that that is going to mean that people who should have been got out sooner are not going to be able to be extracted from Afghanistan," he told reporters on Saturday.

The federal government has pledged to take 3000 Afghan refugees on humanitarian grounds, a commitment dwarfed by those made by Canada, the US, and the UK.

Liberal backbencher John Alexander broke ranks by calling for that to be expanded beyond the 12,000 refugees Australia repatriated during the Syrian civil war. But Mr Robert insisted Australia had been doing "some pretty serious lifting" by resettling another 8500 since 2013.