A 61-year-old man was hospitalised after being hit by a light rail vehicle on Monday morning.
Transport authorities said the incident involved a pedestrian and a tram at the intersection of Northbourne Avenue and Barry Drive at about 9.15am on Monday.
ACT police said it would now investigate the collision.
The man was taken to hospital shortly after the incident in a serious condition, with head and chest injuries, as the light rail driver underwent counselling.
Canberra Health Services would not provide an update on the man's condition.
Monday marked the start of Canberra Metro's Rail Safety Week campaign, encouraging pedestrians, cyclists and motorists to pay attention near the light rail.
Last week, in the lead up to the campaign, the ACT government released footage of dozens of near misses involving the light rail.
Transport minister Chris Steel's spokeswoman said safety was a priority for Canberra Metro.
"Our thoughts are with the person involved," the spokeswoman said.
"The government will continue to monitor improvements to the safety of the transport network."
Emergency services workers, Worksafe officials and Transport Canberra staff were seen at the site a short time after the incident.
The driver used the emergency brakes during the incident, with cracks visible on the tram's windscreen following the collision.
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One person who saw the incident and didn't want to named said the pedestrian had head injuries and "bounced off the tram".
Another witness said they saw the pedestrian on his phone at the time.
"The tram sounded the horn but he kind of froze, and then he was hit," the witness said.
The incident happened near where the first person was hit by a tram in March while the new network was still being tested.
The ACT Emergency Service Agency said an off-duty paramedic nearby was the first person to respond.
In a statement, the agency said paramedics assessed and treated a male pedestrian following the incident.
"The patient was treated at the scene and transported to hospital," it said.
Transport Canberra's director of light rail operations, Ian McGlinn, said Monday's incident was "shocking".
"Any incident on the light rail we consider very serious," Mr McGlinn said.
"Like any incident, we will take the opportunity to review pedestrian movements in the area and see the circumstances around the incident."
Mr McGlinn said visibility was clear when the crash happened, and warned pedestrians to be alert around the light rail.
"It serves as a timely reminder to not be complacent in any way as you interact with the light rail," he said.
"We want people to take responsibility when moving around [the light rail tracks]."
Tram services were stopped in both directions between Alinga Street and Dickson for about two hours. Bus replacement services ran between the two stations, and one northbound lane of Northbourne Avenue was also closed from Alinga Street.
The tram involved remained at the scene until about 10.40am. Safety inspectors and police left the scene a short time later.
Trams resumed normal services just before 11am.