Close to 2000 kangaroos will be culled from 10 sites across the ACT, the territory government confirmed on Friday.

The 'conservation cull' of 1991 eastern grey kangaroos will begin on Monday to protect the biodiversity of grass and woodland areas.

ACT Parks and Conservation director Daniel Iglesias said while a fertility control vaccine is currently being trialled as a way to manage kangaroo numbers, a cull is currently the most "humane" method of controlling the population.

"The numbers to be culled have been based on scientific kangaroo counts in each location. This has been compared to the sustainable carrying capacity for each area that ACT Government ecologists have established by taking into account the habitat requirements of grassland dependent animals and plants," Mr Iglesias said.

The cull comes just weeks after an animal activist was found guilty of hindering last year's shooting operation by blowing a whistle.

Almost 4000 kangaroos have been killed over the past two years as part of the ACT's annual cull.

Previous culls have drawn fierce criticism from animal rights activists due to debate over the animal's "over-abundant" conversation status.

Robyn Soxsmith from the Coalition of Animal Protectors was left shocked and angry by news of the impending cull.

"It's made us more determined to get out there and stop them from shooting these beautiful and peaceful animals," Ms Soxsmith said.

She said the group would "absolutely not" be deterred by the threat of prosecution or additional security measures.

"We're more determined now than ever before to stop this inhumane and cruel massacre of these beautiful animals," she said.

Protesters are also critical of the cull as a land management strategy.

"In my view, having been out there for a number of years to protect these animals, they are not causing any problems at all, in fact they're the actual grassland managers," Ms Soxsmith said.

"We need these animals, not only for the grasslands but also for all of the other animals that inhabit these reserves. They're all a part of the ecosystem. When you take one animal out, it's going to cause problems."

Mr Iglesias warned those who enter the close reserves without permission will face fines.

Warning signs will be placed at all entry points to the reserves and surveillance cameras will be used to enhance public safety and detect illegal activity, he said.

After the cull some of the kangaroo will be used for baits in the ACT Government's wild dog and fox control programs.

The sites where culls will take place include:

These areas - and adjacent unleased land - will be closed to the public while the cull takes place.