Canberra Hospital managers have been accused of physical intimidation and failing to properly investigate allegations of inappropriate behaviour.
It comes months after a damning report from the independent review into the culture of ACT's public health system.
But that review remains veiled in secrecy with the minister and the health departments refusing to disclose areas of concern it was told about.
Community and Public Sector Union says little has changed in many departments since the review with severe cultural issues still rampant.
The union represents a wide range of health workers including administration staff, allied health and some pharmacists.
Regional secretary Madeline Northam called on the Health Minister, Rachel Stephen-Smith, and CEO of Canberra Health Services, Bernadette McDonald, to take immediate action.
She said staff at Canberra Hospital had made a number of extremely serious allegations and Canberra Health Services had failed to act appropriately.
These allegations included physical intimidation and at least one incident where staff were called into a room and told to tell another staff member why they hated them.
"That this sort of behaviour is continuing after the health culture review is astonishing," Ms Northam said.
She said workers did the right thing and reported the issues to human resources who conducted a preliminary assessment of the matters raised.
"Shockingly, Canberra Health Services decided against referring this matter to the Professional Standards Unit for an independent investigation," Ms Northam said.
"If physical intimidation and meetings about hating staff aren't enough to warrant a referral, then what is?"
She said hospital library staff had been instructed by executives that they were not permitted to provide health professionals with more than five journal articles per medical issue.
"When dealing with a complex case, how is a health professional meant to treat a patient effectively with just five articles? That allegation alone should have been enough to warrant a referral to the Professional Standards Unit," she said.
Ms Northam said all preliminary assessments in the organisation should be checked off by an independent representative from another directorate to ensure a level of transparency that was currently lacking.
"There is no doubt that the CEO and the Minister are taking cultural change seriously, but this is not filtering down to middle management," she said.
"Something needs to be done and done quickly."
Australian Medical Association ACT president Antonio Di Dio agreed cultural issues were still a significant problem at the hospital.
He said Ms McDonald and Ms Stephen-Smith had been sincere and proactive in their efforts to improve culture.
Dr Di Dio said while some doctors were optimistic things were changing, others reported no significant change since the culture review.
"We have many doctors that we are supporting and advocating for at this time," he said.
Dr Di Dio said Canberra's GPs were some of the unsung heroes amid the culture and capacity issues that have plagued the system.
"The people that pick up the slack are the small army of hard working GPs in Canberra," he said.
Executive director of people and culture, Janine Hammat, said any allegations of bullying and harassment at Canberra Hospital were taken seriously by management.
She said Canberra Health Services had introduced a new case management approach to identify areas where bullying and harassment occurred.
"Areas identified as having significant issues will have customised interventions developed to address their specific needs as a 'one size fits all approach' will not drive workplace culture improvement," she said.
"People and Culture have worked closely with the employee assistance program to ensure that targeted support is provided to all parties involved.
"[Canberra Health Services] continues to work on strategies and actions to shift the focus from formal investigative processes to early intervention and alternative dispute resolution."
Ms Hammat said managers were given training about the preliminary assessment processes to ensure they were meeting their legal obligations.
She said the health service also had a panel of mediators to help with rebuilding the relationship in the workplace.
"Other Programs will be identified as part of [the health service's] Positive Workplace Strategy currently being developed," Ms Hammat said.