Canberra Hospital's neurology department is one of the first to undergo an overhaul since health's independent review last year.
While Canberra Health Services has refused to disclose hotspots of poor culture identified, it is understood the department was a key area of concern.
Some staff say the significant culture problems have made it difficult to deliver some important clinical services.
Staff were recently notified work would begin on improving the department's culture in an internal email.
"I am ... aware the neurology department has been experiencing a number of issues and challenges impacting the workplace culture and your experience of working in the department," executive director of medicine Jacqui Taylor said in the email.
"I'm committed to addressing these issues and challenges and to do so, need your feedback."
Ms Taylor said a "cultural diagnostic" on what people were thinking and feeling would be undertaken.
Staff were invited to take part in one-on-one or small group discussions with human resources throughout November.
It's understood cultural issues have at times left staff concerned the department is hamstrung in its ability to introduce services despite having the skills to do so.
Some staff say research has been put ahead of delivering important clinical services. There appears little faith the culture review will lead to any real change.
Some are frustrated at the health department's inability to get it up and running - despite qualified doctors and other clinicians being willing to facilitate it.
Currently, patients who need the time-sensitive procedure have to be transferred to Sydney.
Canberra Hospital has promised there will be a 24 hour service by the end of the year but, a month out from that deadline, has not been able to provide a start date.
Canberra's public health system underwent an independent review into its culture last year after widespread concerns about bullying and harassment.
But Canberra Health Services has refused to acknowledge even large areas within the service which were found to be hotspots of poor culture.
Despite the areas being broad and having many staff members, they have continued to defer to "privacy concerns".
A Canberra Health Services spokeswoman said a significant amount of work was underway to implement the culture review's recommendations.
"A 'one size fits all approach' will not, on its own, drive workplace culture improvement," she said.
"With this in mind, Canberra Health Services has introduced a case management approach to assist with identifying where cultural issues might exist in the organisation.
"Where issues are identified, we use customised interventions which are developed to address specific needs.
"[Canberra Health Services] continues to work on strategies and actions to shift the focus from formal investigative processes to early intervention and alternative dispute resolution."
Asked what other departments were going through a cultural diagnosis, the spokeswoman cited privacy concerns.
"To ensure ongoing employee confidentiality, [Canberra Health Services] is not able to disclose information about specific areas," she said.
Meanwhile, in a development that is not understood to be related to the cultural issues, after 16 years in the neurology department, the clinical head has recently announced his resignation. Canberra Health Services says he is helping find a replacement.
Australian Medical Association ACT president Antonio Di Dio said the neurologists were all hard working and dedicated specialists.
He said they had given up countless hours and days to try to get the ECR stroke service up and running.